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91.
92.
In order to obtain a radioimmunoassay (RIA) technique for the measurement of human plasma myeloperoxidase (MPO), we purified the enzyme from polymorphonuclear granulocytes (neutrophils), and compared three methods of labeling it with125Iodine: chloramine T, lactoperoxidase, and an original technique of self labeling based on the ability of the enzyme to oxidize and bind125I in the presence of H2O2. The chloramine T technique produced a degraded protein, as well shown by a high non-specific binding of tracer to antibody. The lactoperoxidase technique did not succeed in labeling MPO with an adequate specific activity. In contrast, the self-labeling method gave a stable tracer with a specific activity of 23 Ci/gmg MPO (85 MBq), a satisfactory level of immunoreactivity, and a low-specific binding (3%). After labeling, purification of tracer was achieved by gel filtration chromatography in phosphate buffer (0.05 M; pH7) to which 0.1% poly-L-lysine was added. The labeled molecule remained stable for 40 days and could be used for RIA with a polyclonal antibody raised in rabbits.  相似文献   
93.
Summary Behavioral arousal and fright responses ofHalichoeres bivittatus occurred in aquaria to a moving shadow and a tap stimulus. Arousal was characterized by changes in the beat of pectoral fins, dorsal fin erection and eye movements, whereas in fright, adduction of pectoral and dorsal fins and rapid forward movement occurred. Serial stimulus presentation caused the fright response to be replaced by arousal which habituated in that the proportion of behavioral components exhibited decreased during the process.P.R. Laming was in receipt of a Royal Society travel grant for assistance in the expenses of this work, for which he is duly grateful. We are grateful to Nick Zinkowski, Culebra for the loan of minnow traps.  相似文献   
94.
We look into the interaction of Google's search queries and several aspects of international equity markets. Using a novel methodology for selecting words and a vector autoregressive modeling approach, we study whether the search queries of finance‐related words can have an impact on returns, volatility of returns and traded volume in four different English‐speaking countries. We identify several words whose search frequency is associated with changes in the dependent variables. In particular, we find that increases in search queries including the word stock predict increased volatility and decreased index returns over the next week. On top of that, we investigate the performance of a market‐timing strategy based on the search frequency of this word and benchmark it against random words from the Word‐Net database and a naive buy‐and‐hold strategy. The results of this empirical application are positive and particularly stronger during the global crisis of 2009. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   
95.
Zusammenfassung Die Goitrogen-Wirkung des Metopirons (SU-4885, CIBA) wurde in Feld- und Wüstenmaus mittels radioaktiven I131 untersucht. Das radioiodingebundene Protein (Pb I131) war nach SU-4885 vermindert. Die Zunahme der gesamten Radioaktivität in der Schilddrüse lag bei 49%. Metopiron wirkt als Schilddrüsen-Hemmer.

We are grateful to Prof.P. N. Srivastava for providing facilities. The investigation was supported by University Grants Commission, New Delhi, India.  相似文献   
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Summary L1210 leukemic cells grown in vitro were subjected to kinetic analysis using a flow microfluorometer. A single broad peak was found for the DNA content distribution if unfractionated cells were used; prior fractionation using lg velocity sedimentation allowed the separation of small peaks with smaller (G1) and larger (G2) DNA contents from the dominant S phase peak with intermediate DNA content.This work was supported by grant number 5P01CA13053 awarded by the National Cancer Institute, DHEW USA, and by grant num ber RBI 76-1 from the Queen Wilhelmina Fund, The Netherlands.  相似文献   
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Summary Several thiols and disulphides have been found able both to shorten the latency phase and to increase the growth of several virus strains in cell cultures.This work has been supported by a grant of Consiglio Nazionale delle Ricerche, Roma (Italy).  相似文献   
100.
Summary Chronic catheterization of aorta and inferior vena cava in dogs did not significantly affect circulating levels of prostaglandins (PGE2 and PGF2a ). Pentobarbital (30 mg/kg i.v.) anaesthesia produced a significant decrease in PGF2a .This work was supported by INSERM (grant No. 76.5.183.5).We wish to thank Miss Laure Eloy and Mrs Annick Soubrier for their technical assistance.  相似文献   
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